Forgot to mention earlier this week that Battle for the Cowl #1 came out (on the cover, that's (L-to-R) Wildcat, Huntress, Nightwing, Batwoman, Catwoman, and Damien Wayne in the top right... Tim Drake is the focus of the issue, but isn't pictured for some reason). This is the follow-up to Batman: R.I.P. and Final Crisis, where Gotham City deals with the absence of its hero. It was pretty good, though if you've been keeping up with the Robin, Nightwing, or Birds of Prey titles, you'll find it irritating that the past few months of plot in all three books seem to have been ignored.
After The Scarecrow, Ra's Al Ghul, The Joker, and Two-Face, who should be Batman's next enemy in the Batman Begins/The Dark Knight series? Well, as of now we know that Tom Hardy will play Bane and Anne Hathaway will play Catwoman.
Check out the master list for all the villains covered. I've pretty much run out by now. Obviously, there are more Batman villains, but not any of significance that I know of. As of February 2009, no one is confirmed as a villain. Not the Johnny Depp Riddler, not Phillip Seymour Hoffman Penguin, nobody.